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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Optimization of Mass Spectrometry Imaging for Drug Metabolism and Distribution Studies in the Zebrafish Larvae Model: A Case Study with the Opioid Antagonist Naloxone
doi: 10.3390/ijms241210076
Figure Lengend Snippet: Comparison of the spatial distribution of naloxone (sodium adduct ion ( m / z 350.136) and potassium adduct ion ( m / z 366.110)) inside ZF larval whole body between ( a , b ) the protocol used in the previous studies [ , ] and ( c , d ) the optimal condition investigated in this study. All MS images were visualized with a three-dimensional view function in SCiLS Lab version 2022a Premium 3D software, based on the diagram of a ZF larva shown in . The experimental conditions were the following: ( a , b ) 14 passes of spraying matrix deposition using 15 mg mL −1 DHB containing 0.1% TFA and 40% gelatin embedding medium, a total of 10 slides for one respective larva; ( c , d ) 8 passes of spraying matrix deposition using 5 mg mL −1 CHCA containing 0.1% TFA and 40% gelatin embedding medium, a total of 11 slides for one respective larva. The images were generated by preparing a color map from blue (no detection) to yellow (high local concentration) with a 50% transparency option, and then they were further processed in 96 dpi resolution with 24-bit color under a denoising state. The red lines indicate individual larval body sections that were overlapped for 3D visualization, and approximately 15 sections were usually utilized for this visualization.
Article Snippet: 2.2.0, flexImaging version 5.0, and
Techniques: Software, Generated, Concentration Assay
Journal: International Journal of Molecular Sciences
Article Title: Optimization of Mass Spectrometry Imaging for Drug Metabolism and Distribution Studies in the Zebrafish Larvae Model: A Case Study with the Opioid Antagonist Naloxone
doi: 10.3390/ijms241210076
Figure Lengend Snippet: MALDI-MS images of the most abundant ZF metabolites of naloxone (M2 ( m / z 326.078), M4 ( m / z 368.125), M7 ( m / z 542.142), M10/M14 ( m / z 382.105), and M35 ( m / z 308.068) potassium adduct ions) detected inside ZF larvae (whole body) prepared under the optimal conditions, as described above. All MS images were visualized with a three-dimensional view function in SCiLS Lab version Premium 2022a 3D software, based on the diagram of a ZF larva shown in . The images were generated by preparing a color map from blue (no detection) to yellow (high local concentration) with a 50% transparency option and then were further processed in 96 dpi resolution with 24-bit color under a denoising state. The red lines indicate individual larval body sections that were overlapped for 3D visualization, and approximately 15 sections were usually utilized for this visualization.
Article Snippet: 2.2.0, flexImaging version 5.0, and
Techniques: Software, Generated, Concentration Assay