version 2022a Search Results


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Comparison of the spatial distribution of naloxone (sodium adduct ion ( m / z 350.136) and potassium adduct ion ( m / z 366.110)) inside ZF larval whole body between ( a , b ) the protocol used in the previous studies [ , ] and ( c , d ) the optimal condition investigated in this study. All MS images were visualized with a three-dimensional view function in SCiLS Lab version <t>2022a</t> Premium 3D software, based on the diagram of a ZF larva shown in . The experimental conditions were the following: ( a , b ) 14 passes of spraying matrix deposition using 15 mg mL −1 DHB containing 0.1% TFA and 40% gelatin embedding medium, a total of 10 slides for one respective larva; ( c , d ) 8 passes of spraying matrix deposition using 5 mg mL −1 CHCA containing 0.1% TFA and 40% gelatin embedding medium, a total of 11 slides for one respective larva. The images were generated by preparing a color map from blue (no detection) to yellow (high local concentration) with a 50% transparency option, and then they were further processed in 96 dpi resolution with 24-bit color under a denoising state. The red lines indicate individual larval body sections that were overlapped for 3D visualization, and approximately 15 sections were usually utilized for this visualization.
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Comparison of the spatial distribution of naloxone (sodium adduct ion ( m / z 350.136) and potassium adduct ion ( m / z 366.110)) inside ZF larval whole body between ( a , b ) the protocol used in the previous studies [ , ] and ( c , d ) the optimal condition investigated in this study. All MS images were visualized with a three-dimensional view function in SCiLS Lab version <t>2022a</t> Premium 3D software, based on the diagram of a ZF larva shown in . The experimental conditions were the following: ( a , b ) 14 passes of spraying matrix deposition using 15 mg mL −1 DHB containing 0.1% TFA and 40% gelatin embedding medium, a total of 10 slides for one respective larva; ( c , d ) 8 passes of spraying matrix deposition using 5 mg mL −1 CHCA containing 0.1% TFA and 40% gelatin embedding medium, a total of 11 slides for one respective larva. The images were generated by preparing a color map from blue (no detection) to yellow (high local concentration) with a 50% transparency option, and then they were further processed in 96 dpi resolution with 24-bit color under a denoising state. The red lines indicate individual larval body sections that were overlapped for 3D visualization, and approximately 15 sections were usually utilized for this visualization.
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Thermo Fisher calc® software version 2022a
Comparison of the spatial distribution of naloxone (sodium adduct ion ( m / z 350.136) and potassium adduct ion ( m / z 366.110)) inside ZF larval whole body between ( a , b ) the protocol used in the previous studies [ , ] and ( c , d ) the optimal condition investigated in this study. All MS images were visualized with a three-dimensional view function in SCiLS Lab version <t>2022a</t> Premium 3D software, based on the diagram of a ZF larva shown in . The experimental conditions were the following: ( a , b ) 14 passes of spraying matrix deposition using 15 mg mL −1 DHB containing 0.1% TFA and 40% gelatin embedding medium, a total of 10 slides for one respective larva; ( c , d ) 8 passes of spraying matrix deposition using 5 mg mL −1 CHCA containing 0.1% TFA and 40% gelatin embedding medium, a total of 11 slides for one respective larva. The images were generated by preparing a color map from blue (no detection) to yellow (high local concentration) with a 50% transparency option, and then they were further processed in 96 dpi resolution with 24-bit color under a denoising state. The red lines indicate individual larval body sections that were overlapped for 3D visualization, and approximately 15 sections were usually utilized for this visualization.
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Comparison of the spatial distribution of naloxone (sodium adduct ion ( m / z 350.136) and potassium adduct ion ( m / z 366.110)) inside ZF larval whole body between ( a , b ) the protocol used in the previous studies [ , ] and ( c , d ) the optimal condition investigated in this study. All MS images were visualized with a three-dimensional view function in SCiLS Lab version <t>2022a</t> Premium 3D software, based on the diagram of a ZF larva shown in . The experimental conditions were the following: ( a , b ) 14 passes of spraying matrix deposition using 15 mg mL −1 DHB containing 0.1% TFA and 40% gelatin embedding medium, a total of 10 slides for one respective larva; ( c , d ) 8 passes of spraying matrix deposition using 5 mg mL −1 CHCA containing 0.1% TFA and 40% gelatin embedding medium, a total of 11 slides for one respective larva. The images were generated by preparing a color map from blue (no detection) to yellow (high local concentration) with a 50% transparency option, and then they were further processed in 96 dpi resolution with 24-bit color under a denoising state. The red lines indicate individual larval body sections that were overlapped for 3D visualization, and approximately 15 sections were usually utilized for this visualization.
Version 9.13 (R2022b), supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Unicon Inc learning tool interoperability (lti; version 1.3) standards
Comparison of the spatial distribution of naloxone (sodium adduct ion ( m / z 350.136) and potassium adduct ion ( m / z 366.110)) inside ZF larval whole body between ( a , b ) the protocol used in the previous studies [ , ] and ( c , d ) the optimal condition investigated in this study. All MS images were visualized with a three-dimensional view function in SCiLS Lab version <t>2022a</t> Premium 3D software, based on the diagram of a ZF larva shown in . The experimental conditions were the following: ( a , b ) 14 passes of spraying matrix deposition using 15 mg mL −1 DHB containing 0.1% TFA and 40% gelatin embedding medium, a total of 10 slides for one respective larva; ( c , d ) 8 passes of spraying matrix deposition using 5 mg mL −1 CHCA containing 0.1% TFA and 40% gelatin embedding medium, a total of 11 slides for one respective larva. The images were generated by preparing a color map from blue (no detection) to yellow (high local concentration) with a 50% transparency option, and then they were further processed in 96 dpi resolution with 24-bit color under a denoising state. The red lines indicate individual larval body sections that were overlapped for 3D visualization, and approximately 15 sections were usually utilized for this visualization.
Learning Tool Interoperability (Lti; Version 1.3) Standards, supplied by Unicon Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Comparison of the spatial distribution of naloxone (sodium adduct ion ( m / z 350.136) and potassium adduct ion ( m / z 366.110)) inside ZF larval whole body between ( a , b ) the protocol used in the previous studies [ , ] and ( c , d ) the optimal condition investigated in this study. All MS images were visualized with a three-dimensional view function in SCiLS Lab version 2022a Premium 3D software, based on the diagram of a ZF larva shown in . The experimental conditions were the following: ( a , b ) 14 passes of spraying matrix deposition using 15 mg mL −1 DHB containing 0.1% TFA and 40% gelatin embedding medium, a total of 10 slides for one respective larva; ( c , d ) 8 passes of spraying matrix deposition using 5 mg mL −1 CHCA containing 0.1% TFA and 40% gelatin embedding medium, a total of 11 slides for one respective larva. The images were generated by preparing a color map from blue (no detection) to yellow (high local concentration) with a 50% transparency option, and then they were further processed in 96 dpi resolution with 24-bit color under a denoising state. The red lines indicate individual larval body sections that were overlapped for 3D visualization, and approximately 15 sections were usually utilized for this visualization.

Journal: International Journal of Molecular Sciences

Article Title: Optimization of Mass Spectrometry Imaging for Drug Metabolism and Distribution Studies in the Zebrafish Larvae Model: A Case Study with the Opioid Antagonist Naloxone

doi: 10.3390/ijms241210076

Figure Lengend Snippet: Comparison of the spatial distribution of naloxone (sodium adduct ion ( m / z 350.136) and potassium adduct ion ( m / z 366.110)) inside ZF larval whole body between ( a , b ) the protocol used in the previous studies [ , ] and ( c , d ) the optimal condition investigated in this study. All MS images were visualized with a three-dimensional view function in SCiLS Lab version 2022a Premium 3D software, based on the diagram of a ZF larva shown in . The experimental conditions were the following: ( a , b ) 14 passes of spraying matrix deposition using 15 mg mL −1 DHB containing 0.1% TFA and 40% gelatin embedding medium, a total of 10 slides for one respective larva; ( c , d ) 8 passes of spraying matrix deposition using 5 mg mL −1 CHCA containing 0.1% TFA and 40% gelatin embedding medium, a total of 11 slides for one respective larva. The images were generated by preparing a color map from blue (no detection) to yellow (high local concentration) with a 50% transparency option, and then they were further processed in 96 dpi resolution with 24-bit color under a denoising state. The red lines indicate individual larval body sections that were overlapped for 3D visualization, and approximately 15 sections were usually utilized for this visualization.

Article Snippet: 2.2.0, flexImaging version 5.0, and SCiLS Lab version 2022a Premium 3D software (Bruker Daltonics, Bremen, Germany).

Techniques: Software, Generated, Concentration Assay

MALDI-MS images of the most abundant ZF metabolites of naloxone (M2 ( m / z 326.078), M4 ( m / z 368.125), M7 ( m / z 542.142), M10/M14 ( m / z 382.105), and M35 ( m / z 308.068) potassium adduct ions) detected inside ZF larvae (whole body) prepared under the optimal conditions, as described above. All MS images were visualized with a three-dimensional view function in SCiLS Lab version Premium 2022a 3D software, based on the diagram of a ZF larva shown in . The images were generated by preparing a color map from blue (no detection) to yellow (high local concentration) with a 50% transparency option and then were further processed in 96 dpi resolution with 24-bit color under a denoising state. The red lines indicate individual larval body sections that were overlapped for 3D visualization, and approximately 15 sections were usually utilized for this visualization.

Journal: International Journal of Molecular Sciences

Article Title: Optimization of Mass Spectrometry Imaging for Drug Metabolism and Distribution Studies in the Zebrafish Larvae Model: A Case Study with the Opioid Antagonist Naloxone

doi: 10.3390/ijms241210076

Figure Lengend Snippet: MALDI-MS images of the most abundant ZF metabolites of naloxone (M2 ( m / z 326.078), M4 ( m / z 368.125), M7 ( m / z 542.142), M10/M14 ( m / z 382.105), and M35 ( m / z 308.068) potassium adduct ions) detected inside ZF larvae (whole body) prepared under the optimal conditions, as described above. All MS images were visualized with a three-dimensional view function in SCiLS Lab version Premium 2022a 3D software, based on the diagram of a ZF larva shown in . The images were generated by preparing a color map from blue (no detection) to yellow (high local concentration) with a 50% transparency option and then were further processed in 96 dpi resolution with 24-bit color under a denoising state. The red lines indicate individual larval body sections that were overlapped for 3D visualization, and approximately 15 sections were usually utilized for this visualization.

Article Snippet: 2.2.0, flexImaging version 5.0, and SCiLS Lab version 2022a Premium 3D software (Bruker Daltonics, Bremen, Germany).

Techniques: Software, Generated, Concentration Assay